Analytical technology

  • Title Pep-MS assay: Protease hydrolysis assay system using photo-cleavable peptide array and mass spectrometer
    Author M.Kurimoto, K.Shimizu*1, H.Ochi, F.Abe, and H.Honda*1,2
    *1Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, *2Innovative Research Center for Preventative Medical Engineering, Nagoya University
    Journal Journal of Bioscience and Bioengineering 128(2): 156-161(2019)
    Summary We have developed a "Pep-MS assay" system that could be used to comprehensively clarify the positions of protease cleavage. The Pep-MS assay can identify a lot of enzyme cleavage sites at once, and the data obtained using this technology is useful for efficiently producing bioactive peptides.