Functional Ingredients

  • Title Allergenicity of partially hydrolyzed whey and casein formulas evaluated by ImmunoCAP inhibition assay and basophil activation test
    Author T.Matsubara, F.Ishikawa, C.Inuo*, M.Fujita*, A.Tsukahara*, T.Koyama, H.Iwamoto, and K.Miyaji
    *Department of Allergy, Kanagawa Children's Medical Center
    Journal Frontiers in Allergy, volume 4: :1207924 (2023)
    Summary Some children develop allergic symptoms, including anaphylaxis, after the first intake of dairy product. Therefore, the tolerability of partially hydrolyzed formula (PHF), used often as a starter formula, in infants with cow’s milk allergy is important information. In this study, we used the blood of children with cow’s milk allergy and showed the tolerability of the PHFs, PHP and E-akachan, developed by our company in Japan or Indonesia is equivalent or better compared to a PHF distributed globally and characterized well.
  • Title Effects of bovine lactoferrin on the maintenance of respiratory and systemic physical conditions in healthy adults-A randomized, double-blind, placebo-controlled trial
    Author H.Oda, S.Kubo, A.Tada, T.Yago, C.Sugita*1, H.Yoshida*1, T.Toida*2, M.Tanaka, and M.Kurokawa*1
    *1Department of Biochemistry, Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare, *2School of Pharmaceutical Sciences, Kyushu University of Health and Welfare
    Journal Nutrients 15(18):3959 (2023)
    Summary Healthy adults ingested lactoferrin (200mg/day) or placebo for 12 weeks. As a result, the total scores for respiratory and systemic symptoms during the intervention were significantly lower in the lactoferrin group than in the placebo group. The expression of activity markers of plasmacytoid dendritic cells was significantly higher in the lactoferrin group than in the placebo group at week 12. These results suggest that orally ingested lactoferrin supports the normal immune system via maintaining the activity of plasmacytoid dendritic cells, and maintains respiratory and systemic physical conditions in healthy adults.
  • Title Whey protein hydrolysate mitigates both inflammation and endotoxin tolerance in THP-1 human monocytic leukemia cells
    Author F.Ishikawa, T.Matsubara, T.Koyama, H.Iwamoto, ans K.Miyaji
    Journal Immunity, Inflammation and Disease 10(12):e737 (2022)
    Summary Severe inflammation and subsequent immunosuppression in critically ill patients is associated with a poor prognosis. In this study, we demonstrated using a human monocytic cell line that whey protein hydrolysate suppresses inflammation and endotoxin tolerance mimicking immunosuppression.
  • Title Lactoferrin and its digestive peptides induce interferon-α production and activate plasmacytoid dendritic cells ex vivo
    Author S.Kubo, M.Miyakawa, A.Tada, H.Oda, H.Motobayashi*1, S.Iwabuchi*2, S.Tamura*3, M.Tanaka, and S.Hashimoto*2
    *1Second Department of Surgery, Wakayama Medical University, *2Department of Molecular Pathophysiology, Wakayama Medical University, *3Department of Hematology/Oncology, Wakayama Medical University,
    Journal Biometals (2022) Aug 26. Online ahead of print.
    Summary Bovine lactoferrin and its pepsin (gastric digestive enzyme) hydrolysate enhanced interferon (IFN)-α production by peripheral blood mononuclear cells, and activated plasmacytoid dendritic cells, an important IFN-α producer, in the presence of viral single-stranded RNA
  • Title Lactulose ingestion induces a rapid increase in genus Bifidobacterium in healthy Japanese: A randomised, double-blind, placebo-controlled crossover trial
    Author Y.Sakai, H.Ochi, and M.Tanaka
    Journal Microorganisms 10(9): 1719 (2022)
    Summary A clinical trial in which 36 healthy Japanese men and women consumed 4 g/day of lactulose resulted in a significant increase in bifidobacteria. The higher bifidobacterial occupancy was observed 2 days after starting lactulose ingestion and maintained until the end of lactulose intervention after 2 weeks.
  • Title Time courses of gastric volume and content after different types of casein ingestion in healthy men: A randomized crossover study
    Author Y.Sakata, T.Yago, S.Mori*1, N.Seto, Y.Matsunaga*2, H.Nakamura, T.Tominaga*3 K.Miyaji, and Y.Takeda
    *1Department of Physiological Laboratory, Japanese Red Cross Sagamihara Hospital, *2Department of Sports Sciences, The University of Tokyo, *3Tominaga Internal Medicine Clinic
    Journal The Journal of Nutrition 152(11): 2367–2375 (2022)
    Summary We evaluated gastric emptying and plasma amino acid absorption kinetics after milk protein ingestion in a cross-over study of ten healthy men. Compared with sodium caseinate and whey protein, gastric emptying and plasma amino acid absorption kinetics after micellar casein ingestion showed slow. We also observed that the slow kinetics after micellar casein ingestion were affected by curd formation in the stomach.
  • Title Novel health function of Aloe vera gel-derived phytosterols and their application in functional foods(JSNFS Award for Technology and Innovation (2018))
    Author E.Misawa, M.Tanaka, F.Abe, K.Yamauchi, M.Saito, and K.Nabeshima
    Journal Journal of Japan Society of Nutrition and Food Sciences 72(4): 141-145 (2019)
    Summary We have identified five phytosterols (Aloe sterol) obtained from Aloe vera gel as functional ingredients. We investigated the effects on skin function by basic and clinical studies. From these studies, it was confirmed that the usefulness of Aloe sterol on maintenance and promotion of skin health as functional food material.
  • Title Prebiotic effect of two grams of lactulose in healthy Japanese women: a randomised, double-blind, placebo-controlled crossover trial
    Author Y.Sakai, N.Seki, H.Hamano, H.Ochi, F.Abe, K.Masuda*1, and H.Iino*2
    *1Department of Food Science and Nutrition, Faculty of Life and Environmental Sciences, Showa Women’s University, *2Life Sciences for Living Systems, Graduate School, Showa Women’s University
    Journal Beneficial Microbes 10(6): 629-639 (2019)
    Summary In a randomised, double-blind crossover trial with 60 healthy Japanese women, the defaecation frequency, faecal consistency, faecal volume, and the number and percentage of Bifidobacterium in faeces were significantly higher during 2 g/day lactulose than placebo, but flatulence was unchanged.
  • Title A study of the prebiotic effect of lactulose at low dosages in healthy Japanese women
    Author Y.Sakai, N.Seki, H.Hamano, H.Ochi, F.Abe, F.Shimizu*1, K.Masuda*1, and H.Iino*2
    *1Department of Food Science and Nutrition, Faculty of Life and Environmental Sciences, Showa Women’s University, *2Life Science for Living System, Graduate School, Showa Women’s University,
    Journal Bioscience of Microbiota, Food and Health 38(2): 69-72 (2019)
    Summary We assessed changes in defaecation frequency following ingestion of 1, 2, or 3 g/day of lactulose for 2 weeks. This was an open-label, before-after trial that enrolled 26 healthy Japanese women. The defaecation frequency, number of defaecation days, and number of faecal bifidobacteria increased significantly compared with before ingestion of 1, 2, and 3 g/day of lactulose.
  • Title Casein materials show different digestion patterns using an in vitro gastrointestinal model and different release of glucagon-like peptide-1 by enteroendocrine GLUTag cells
    Author Y.Komatsu, Y.Wada, H.Izumi, T.Shimizu, Y.Takeda, T.Hira*, and H.Hara*
    *Research Faculty of Agriculture, Hokkaido University
    Journal Food Chemistry 277: 423-431 (2019)
    Summary We compared protein digestibility and glucagon-like peptide-1 (GLP-1) -releasing activities of micellar casein concentrate (MCC) and sodium caseinate (SCN). The digestibility of MCC was higher than that of SCN. A comparison of GLP-1-releasing activities showed that MCC induced GLP-1 secretion to a greater extent than SCN.
  • Title Investigation of potent odorants generated during the production
    Author H.Nakada, M.Ohata*1,2, M.Hosaka*1, H.Ochi, F.Abe, and K.Arihara*1
    *1Kitasato University, *2Nihon University
    Journal Animal Science Journal 89(9):1348-1354(2018)
    Summary We evaluated predominant odorants of Whey protein hydrolysates(WPH), and our results showed that difference of odor characteristics between whey protein concentrate(WPC) and WPH, and three odor-active compounds contributed to the odor of WPH. Additionally, this study suggests that specific free amino acids are important precursors for generation of these compounds in manufacturing process of WPH.
  • Title Antihypertensive effect of the casein-derived peptide Met-Lys-Pro in individuals with high-normal blood pressure or grade 1 hypertension-A randomized, double-blind, placebo-controlled, parallel-group trial-
    Author N.Yuda, M.Tanaka, A.Yamada, D.Ochi, K.Yamauchi, F.Abe, and N.Sakane*
    *National Hospital Organization Kyoto Medical Center
    Journal Japan Pharmacology & Therapeutics 46(4):529-37(2018)
    Summary Antihypertensive effect of the casein-derived peptide Met-Lys-Pro (MKP) was confirmed by clinical trial. Daily MKP ingestion is a effective systolic blood pressure lowering treatment for people with mildly high blood pressure.
  • Title Blood pressure-lowering effects of casein-derived peptide met-lys-pro in subjects with high-normal blood pressure and grade 1 hypertension - A randomized, double-blind, placebo-controlled, parallel-group, trial-
    Author D.Ochi, A.Yamada, K.Yamauchi, F.Abe, and Y.Tho*
    *Aiwa Clinic, Latvia
    Journal Japanese pharmacology & therapeutics 45(10): 1637-1648 (2017)
    Summary We evaluated the effect of Met-Lys-Pro(MKP) in lowering blood pressure in subjects with high-normal blood pressure and grade 1 hypertension in a randomized, double-blind, placebo-controlled, parallel-group trial for 12 weeks.
    The result showed that 100 μg MKP has a beneficial effect on lowering systolic and diastolic blood pressure.
  • Title Effects of lactoferrin and lactoperoxidase-containing food on the oral microbiota of older individuals
    Author M.Nakano, H.Wakabayashi, H.Sugahara, T.Odamaki, K.Yamauchi, F.Abe, JZ.Xiao, K.Murakami*, K.Ishikawa*, and S.Hironaka*
    *Department of Special Needs Dentistry, Division of Hygiene and Oral Health, Showa University School of Dentistry
    Journal Microbiology and Immunology 61(10): 416-426 (2017)
    Summary The long-term ingestion of lactoferrin and lactoperoxidase-containing tablets promotes a shift from a gram-negative-dominated unhealthy community to a gram-positive-dominated healthy community in the oral microbiota.