Articles

Milk and Dairy Products

Title Development of a Rapid and Accurate Prediction Model for Whey Separation in Pasteurized Drinking Yogurt Caused by Long-term Ambient Storage
Author K.Miyaji, H.Maruyama, Y.Kuwano, Y.Katakura, H.Inoue, and N.Azuma*
*Department of Applied Biochemistry, Faculty of Agriculture, Utsunomiya University
Journal Food Science and Technology Research 26(6): 863-873 (2020)
Summary We newly found the Turbiscan Stability Index model that allows for more rapid, accurate and versatile evaluation of spontaneous whey separation of long-term ambient storage of pasteurized drinking yogurt as compared to models based on other physical property measurements.
Title Prediction of physiological functions of pasteurized yogurt using Capillary Electrophoresis Time-of-Flight Mass Spectrometry
Author K.Miyaji, Y.Kuwano, H.Maruyama, H.Inoue, and N.Azuma*
*Department of Applied Biochemistry, Faculty of Agriculture, Utsunomiya University
Journal Milk Science 69(3): 135-145 (2020)
Summary We confirmed that the pasteurization process after fermentation is involved in the physiological functions of pasteurized yogurt by analyzing trace components, including metabolites in fresh and pasteurized yogurt. Our results suggest that this pasteurization may positively affect yogurt’s physiological function.
Title Flavor compounds affecting the sensory characteristics of a Lactobacillus-fermented dairy beverage during two weeks of refrigerated storage
Author T.Suzuki, M.Akiyama, Y.Sato, M.Okaue, Y.Murakami*, M.Onishi*, Y.Mizota, H.Ochi, R.Koizumi, K.Miyaji, M.Ikeda, and H.Iwabuchi*
*San-Ei Gen F.F.I., Inc.
Journal Food Science and Technology Research 26(1): 139-152 (2020)
Summary Lactobacillus paracasei MCC1849 (LP) has the potential to modulate immune function. Flavor compounds related to changes in sensory characteristics during refrigerated 2-week storage of a functional LP-fermented dairy beverage were investigated by partial least squares regression analysis between analytical sensory evaluation and GC-MS data.
Title Sensory evaluation and taste sensor analysis of milk coffee drinks manufactured under different processing conditions
Author M.Ikeda, Y.Hirano, M.Akiyama, K.Miyaji, K.Takahashi, R.Koizumi, T.Onodera*, and K.Toko*
*Information Science and Electrical Engineering of Kyushu University
Journal Research reports on Information Science and Electrical Engineering of Kyushu University 24(2): 23-28 (2019)
Summary As the manufacturing process of a ready-to-drink milk coffee that retains flavor characteristics similar to a homemade milk coffee, it was showed that a new process without pH adjustment of coffee extract was superior to a conventional process with pH adjustment of coffee extract, by the investigation using sensory evaluation and taste sensor analysis.
Title Influences of beating speed on whipping and physical properties of whipped cream
Author K.Ihara, Y.Nishimura, K.Habara, and F.Abe 
Journal Food Science and Technology Research 25(3): 391-397 (2019)
Summary Change in beating speed in the middle of whipping process brought short whipping time and high shape-retention ability to whipped cream. In this whipping condition, the size of air bubble in whipped cream became smaller than regular ones, thus the small bubble size could be one of the reasons for this effect.
Title Predicting sensory evaluation indices of Cheddar cheese texture by fluorescence fingerprint measurement coupled with an optical fibre
Author A.Chiba, M.Kokawa*1, M.Tsuta*2, and S.Todoriki*1,2
*1Faculty of Life and Environmental Sciences, University of Tsukuba, *2Food Research Institute, National Agriculture and Food Research Organisation
Journal International Dairy Journal 91: 129-136 (2019)
Summary A fluorescence fingerprint (FF) was used to develop a quick and non-contact practical method for predicting the sensory evaluation index of cheese texture (cheese body measurements). A partial least squares model was constructed from FF data and cheese body measurements of Cheddar cheeses. The cheese body measurement was successfully predicted by the PLS model with a coefficient of determination for calibration of 0.800.
Title Effects of manufacturing conditions on the foaming properties of milk and sensory characteristics of foamed milk
Author S.Hatakeyama, M.Akiyama, R.Yoneyama, K.Watanabe, R.Koizumi, K.Miyaji, Y.Mizota, M.Ikeda, and S.Wakao
Journal LWT Food Science and Technology, 99: 555-561(2019)
Summary This study showed that different manufacturing conditions of milk had significant effects on the foaming properties and sensory characteristics of foamed milk. The foaming properties were investigated using a Dynamic Foam Analyzer 100 and the sensory characteristics of foamed milk were evaluated by descriptive analysis by means of a trained panel.
Title Effects of homogenization pressure on sensory characteristics, preference and flavor release of flavored dairy beverages
Author T.Kawaguchi
Journal Journal of Japan Asssociation on Odor Environment 50(2): 130-137(2019) 
Summary Homogenization pressure for producing milk or dairy beverages was investigated to affect significantly on these sensory characteristics, preference and flavor release by sensory evaluation and gas chromatography-mass spectrometry (GC-MS) analysis using a retronasal aroma simulator (RAS).
Title Effects of manufacturing processing conditions on retronasal-aroma odorants from a milk coffee drink
Author M.Ikeda, M.Akiyama, Y.Hirano, K.Miyaji, Y.Sugawara*1, Y.Imayoshi*1, H.Iwabuchi*1, T.Onodera*2, and K.Toko*2
*1San‐Ei Gen F.F.I., Inc., *2Kyusyu Univ.
Journal Journal of Food Science 83(11): 2733-2744 (2018)
Summary From GC-O analysis of the retronasal-aroma odorants from a milk coffee drink, it is revealed that the new manufacturing process without pH adjustment of coffee is superior to the conventional process with pH adjustment in retaining the coffee original aroma.
Title Changes in odor compounds of a Lactobacillus-fermented dairy beverage during two weeks of refrigerated storage
Author M.Akiyama, T.Suzuki, Y.Murakami*, M.Onishi*, Y.Mizota, K.Miyaji, M.Ikeda, and H.Iwabuchi*
*San-Ei Gen F.F.I., Inc.,
Journal Food Science and Technology Research, 24 (6): 1129-1137 (2018)
Summary Lactobacillus paracasei MCC1849 (LP) has the potential to modulate immune function. Changes in odor compounds of a functional LP-fermented dairy beverage during two weeks of refrigerated storage were analyzed by gas chromatography-olfactometry. Thirty-four odorants were detected, of which 29 were identified. 4-(4’-Hydroxyphenyl)-2-butanone (raspberry ketone) was firstly identified as an odorant in fermented dairy products.
Title Effects of Processing Conditions During Manufacture on Retronasal-Aroma Compounds from a Milk Coffee Drink
Author M.Ikeda, M.Akiyama, Y.Hirano, K.Miyazi, M.Kono*1, Y.Imayoshi*1, H.Iwabuchi*1, T.Onodera*2, and K.Toko*2
*1 San-Ei Gen F.F.I., Inc., *2Kyushu Univ.
Journal Journal of Food Science 83(3): 605-616 (2018)
Summary Regarding the manufacture of a ready-to-drink (RTD) milk coffee that retained original volatile characteristics, an investigation of retronasal-aroma compounds from an RTD milk coffee revealed that a new process without pH adjustment of coffee was superior to a conventional process with the pH adjustment.

Nutrition

Title Comparison of isolation methods using commercially available kits for obtaining extracellular vesicles from cow milk
Author M.Morozumi, H.Izumi, T.Shimizu, and Y.Takeda
Journal Journal of Dairy Science 104(6): 6463-6471 (2021)
Summary Milk-derived Extracellular vesicles (EV) are widely known because of their potential for immune enhancement. However, procedures for isolating milk-derived EV have not been fully established. A comparison of isolation methods for obtaining milk-derived EV showed that a combination of acetic acid precipitation and size exclusion chromatography can effectively isolate high amounts of pure EV from bovine milk.
Title Serum albumin redox states: more than oxidative stress biomarker
Author F.Tabata, Y.Wada, S.Kawakami, and K.Miyaji
Journal Antioxidants 10(4): 503 (2021)
Summary Serum albumin redox state has long been viewed as a biomarker of systemic oxidative stress, but recent studies revealed oxidized albumin per se could aggravate the pathological conditions. Furthermore, serum albumin redox state has been demonstrated as a sensitive protein nutrition biomarker. This article provides an updated overview of analytical techniques for serum albumin redox state and its association with human health, focusing on recent findings.
Title Relationship Between Blood Test Values, Including Blood Glucose and Uric Acid, and Lifestyle in Adults ―A Cross-sectional Study in Matsumoto, Japan―
Author D.Ochi, K.Nomaguchi, M.Tanaka, F.Abe, I.Kakiuchi*1, K.Kiyosawa*1, M.Miyasaka*1, M.Nakamura*2, and N.Sakane*3
*1Matsumoto Junior College, *2Matsumoto City Hospital, *3Division of Preventive Medicine, Clinical Research Institute, National Hospital Organization Kyoto Medical Center
Journal Japanese Pharmacology & Therapeutics 48(6): 969-978 (2020)
Summary As a result of investigation of blood test values, including blood glucose and uric acid, and lifestyle in adults in Matsumoto, it was found that lifestyle factors such as weight gain, drinking, dairy product intake, and fruit intake were each associated with blood test values.

Bifidobacteria and Intestinal Flora

Title Bifidobacterium response to lactulose ingestion in the gut relies on a solute-binding protein-dependent ABC transporter
Author K.Yoshida, R.Hirano*1,2, Y.Sakai, M.Choi*3, M.Sakanaka*3,4, S.Kurihara*2,4, H.Iino*5, J.Z.Xiao, T.Katayama*3,4, and T.Odamaki
*1Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, *2 Biology-Oriented Science and Technology, Kindai University, *3 Graduate School of Biostudies, Kyoto University, *4 Faculty of Bioresources and Environmental SSciences, Ishikawa Prefectural University, *5 Life Science for Living System, Graduate School, Showa Women’s University
Journal Communications Biology 4(1): Article number 541 (2021)
Summary We identified a substrate-binding protein involved in the utilization of lactulose using mutant strains of Bifidobacterium. Bifidobacteria carrying this gene were detected from 89% of Japanese gut microbiome.The amount of this gene in the gut microbiota make difference in the growth of bifidobacteria by lactulose intake. This syudy showss the gene could be a maker for predicting subjects with the potential for marked increase in bifidobacterial abundance inlactulose intake.
Title Association of plasma hemoglobin A1c with improvement of cognitive functions by Probiotic Bifidobacterium breve supplementation in healthy adults with mild cognitive impairment
Author F.Bernier, K.Ohno, N.Katsumata, T.Shimizu, and Jz.Xiao
Journal Journal of Alzheimer's Disease 81(2): 493-497 (2021)
Summary To understand the mechanism of B. breve MCC1274, which has been reported to improve cognitive function, we analyzed the correlation between blood parameters and mental function changes (RBANS) and found an inverse correlation with HbA1c. In a stratified analysis based on HbA1c, the effect of B. breve MCC1274 was more apparent in the higher HbA1c group.
Title Effects of Heat-killed Lactobacillus helveticus MCC1848 Intake on QOL
Author N.Mutoh, A.Hiraku, K.Watanabe, N.Iwabuchi, M.Tanaka, F.Abe, and T.Tominaga*
*Tominaga Clinic
Journal Japanese Pharmacology & Therapeitics 49(4):605-612 (2021)
Summary We investigated the change in quality of life (QOL) of healthy adults with slightly high levels of fatigue by consuming heat-killed Lactobacillus helveticus MCC1848. The results showed that mood, sleep, and fatigue conditions were improved. This study suggests that the heat-killed lactic acid bacteria can improve QOL.
Title Growth-promoting effect of alginate on Faecalibacterium prausnitzii through cross-feeding with Bacteroides
Author R.Murakami, N.Hashikura, K.Yoshida, JZ.Xiao, and T.Odamaki
Journal Food Reseach International 144: 110326 (2021)
Summary The nine candidates of prebiotics for Faecalibacterium prausnitzii that is butyrate producer were evaluated using a pH-controlled single-batch fermenter as a human gut microbiota model. Of them, alginate markedly increased the relative abundance of F. prausnitzii. Nevertheless, F. prausnitzii strains were incapable of utilizing alginate in monoculture. The co-culture with Bacteroides revealed that some F. prausnitzii strains assimilate Bacteroides-degraded alginate oligomers.
Title Enriched metabolites that potentially promote age-associated diseases in subjects with an elderly-type gut microbiota
Author S.Yoshimoto, E.Mitsuyama, K.Yoshida, T.Odamaki, and JZ.Xiao
Journal Gut Microbes 13(1): 1-11 (2021)
Summary Fecal metabolites in Japanese healthy elderly subjects, who fell into an age-matched or an age-mismatched cluster, were measured. We found some metabolites elevated in the aged gut microbiota were metabolites reported as risk factors for age-associated diseases such as arteriosclerosis and colorectal cancer. These findings suggest that metabolites produced by the aged gut microbiota could contribute to intestinal and systemic homeostasis and could be targeted for preventing aging-associated diseases.
Title Probiotic Bifidobacterium breve in improving cognitive functions of older adults with suspected mild cognitive impairment: A randomized, double-blind, placebo-controlled trial
Author Jz.Xiao, N.Katsumata, F.Bernier, K.Ohno, Y.Yamauchi, T.Odamaki, K.Yoshikawa*1, K.Ito*2, and T.Kaneko*3
*1Huma R & D Co. Ltd, *2Nihonbashi Sakura Clinic, *3Tokyo Skytree Station Medical Clinic
Journal Journal of Alzheimer's Disease 77(1): 139-147 (2020)
Summary In a randomized, double-blind, placebo-controlled trial, 80 healthy older adults suffering from mild cognitive impairment (MCI) were administrated either probiotic (B. breve A1 (MCC1274)) or placebo for 16 weeks. Significant and remarked improvement of cognitive functions was observed when assessed by RBANS (the primary endpoint) and JMCIS (the secondary endpoint). The study indicate B. breve A1 is a safe and effective approach for improving memory functions of MCI subjects. 
Title Potential effects of indole-3-Lactic Acid, a metabolite of human Bifidobacteria, on NGF-induced neurite outgrowth in PC12 cells
Author CB.Wong, A.Tanaka, T.Kuhara, and JZ.Xiao
Journal Microorganisms 8(3). Pii: E398 (2020)
Summary Gut microbiota-derived tryptophan metabolites could mediate gut-brain communication. Infant-type Human-Residential Bifidobacteria (HRB) produced higher levels of indole-3-lactic acid (ILA). We found that ILA enhanced NGF-induced neurite outgrowth of PC12 cells in a dose-dependent manner. ILA potentiated neurite outgrowth through the Ras/ERK pathway, and also in an aryl hydrocarbon receptor (AhR)-mediated manner. These new findings provide clues into the potential involvement of ILA as the mediator in bifidobacterial host-microbiota crosstalk and neuronal developmental processes.
Title Production of indole-3-lactic acid by Bifidobacterium strains isolated from human infants
Author T.Sakurai, T.Odamaki, and JZ.Xiao
Journal Microorganisms 7(9): 340 (2019)
Summary We examined the production of Indole-3-lactic acid (ILA) by Bifidobacterium strains found in the gastrointestinal tracts of humans and other animals. Strains of bifidobacterial species commonly isolated from the intestines of human infants, such as Bifidobacterium longum subsp. longum, Bifidobacterium longum subsp. infantis, Bifidobacterium breve, and Bifidobacterium bifidum, produced higher levels of ILA than did strains of other species.
Title Association between blood omega-3 polyunsaturated fatty acids and the gut microbiota among breast cancer survivors
Author A.Horigome, R.Okubo*1, K.Hamazaki*2, T.Kinoshita*3, N.Katsumata, Y.Uezono*4, J.Z.Xiao, and Y.J.Matsuoka*1
*1 Division of Health Care Research, Center for Public Health Science, National Cancer Center Japan *2Department of Public Health, Faculty of Medicine, University of Toyama *3Department of Breast Surgery, National Cancer Center Hospital *4Division of Cancer Pathophysiology, National Cancer Center Research Institute
Journal Beneficial Microbes 10(7): 751-758 (2019)
Summary We conducted a cross-sectional study to examine the association between blood polyunsaturated fatty acids (PUFAs) and the gut microbiota among breast cancer survivors. Multiple regression analysis controlling for possible confounders indicated that an increased relative abundance of Bifidobacterium was significantly associated with increased levels of docosahexaenoic acid (DHA).
Title Effects of Bifidobacterium breve A1 on the cognitive function of older adults with memory complaints: a randomised, double-blind, placebo-controlled trial.
Author Y.Kobayashi, T.Kuhara, M.Oki*, and Jz.Xiao
*Seishukai Clinic
Journal Beneficial Microbes 10(5):511-520 (2019)
Summary Intakes of B. breve A1 for 12 weeks showed a significant improvement in the immediate memory function and MMSE score compared to the placebo group in elderly people with low cognitive function index.
Title Neonatal oral fluid as a transmission route for bifidobacteria to the infant gut immediately after birth.
Author K.Toda, K.Hisata*1, T.Satoh*2, N.Katsumata, T.Odamaki, E.Mitsuyama, T.Katayama*3, T.Kuhara, K.Aisaka*4, T.Shimizu*1, and Jz.Xiao
*1Department of Pediatrics and Adolescent Medicine, Juntendo University Graduate School of Medicine, *2Department of Molecular Microbiology, Tokyo University of Agriculture, *3Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, *4Department of Obstetrics and Gynecology, Hamada Hospital
Journal Scientific Reports 9(1):8692 (2019)
Summary We found viable Bifidobacterium in neonatal oral fluid just after birth, and some of them were detected in corresponding infant feces at one month after birth. These results suggest that Bifidobacterium can be transmitted to neonatal oral fluid just after birth, followed by colonizing infant gut.
Title Impact of a bathing tradition on shared gut microbe among Japanese families
Author T.Odamaki, F.Bottacini*, E.Mitsuyama, K.Yoshida, K.Kato, JZ.Xiao, and D.van Sinderen*
*APC Microbiome Ireland and School of Microbiology, National University of Ireland
Journal Scientific Reports 9(1): Art.No. 4380 (2019)
Summary Sharing of Bifidobacterium longum strains had recently been shown to occur among Japanese family members, a phenomenon that is not confined to mother-infant pairs. In the current study, we investigated if bathtub water is a possible vehicle for the exchange of strains as a consequence of a Japanese custom to share bathtub water by family members during bathing practices. Viable bifidobacterial strains were isolated from all bathtub water samples. We showed that the fecal microbiota composition among family members that share bathtub water is significantly closer than that between family members that do not engage in this practice. Our results indicate that bathtub water represents a vehicle for the transmission of gut bacteria, and that the Japanese custom of sharing bathtub water contributes to the exchange of gut microbes, in particular bifidobacteria, among family members.
Title Bifidobacterium breve A1 supplementation improved cognitive decline in older adults with mild cognitive impairment: an open-label, single-arm study
Author Y.Kobayashi, T.Kinoshita*1, A.Matsumoto*2, K.Yoshino*2, I.Saito*1, and JZ.Xiao
*1Ehime Univ. *2Aki Orthopedic Rehabilitation Clinic
Journal The Journal of Prevention of Alzheimer's Disease 6(1):70-75 (2019)
Summary We conducted an open-label, single-arm study to examine the effects of 24-week supplementation of B. breve A1 on 19 elderly with mild cognitive impairment(MCI). The study showed that oral supplementation of B. breve A1 in participants with MCI improved cognitive function.
Title Association between functional lactase variants and a high abundance of Bifidobacterium in the gut of healthy Japanese people
Author K.Kato, S.Ishida*, M.Tanaka*, E.Matsuyama, Jz.Xiao, and T.Odamaki
*1Business Planning Group, Next Generation Science Institute, DeNA Life Science, Inc., *2RandD Group, DeNA Life Science, Inc.
Journal PLoS ONE 13(10), e0206189(2018)
Summary We investigated the possible contribution of functional lactase loci to the high abundance of Bifidobacterium in Japanese populations. The results indicated low lactase activity in Japanese people and possible contribution of host genetic variation-associated low lactase activity to the high abundance of Bifidobacterium in the Japanese population.
Title Breast milk on the infant faecal microbiome: Components of breast milk orchestrating the establishment of bifidobacteria species.
Author C.B.Wong and Jz.Xiao
Journal Journal of Human Nutrition & Food Science 6(2): 1123 (2018)
Summary In this review, we introduced the reason why only human bifidobacteria proliferate in the intestine of babies, focusing on their compatibility with oligosaccharides and lysozyme in breast milk.
Title Effects of paraprobiotic Lactobacillus paracasei MCC1849 supplementation on symptoms of the common cold and mood states in healthy adults
Author M.Murata, J.Kondo*1, N.Iwabuchi, S.Takahashi, K.Yamauchi, F.Abe, and K.Miura*2
*1 Department of Food and Nutrition, Higashi Chikushi Junior College, *2 Department of Nutrition, Faculty of Home Economics, Kyushu Women’s University
Journal Beneficial Microbes 9 (6): 855-864 (2018)
Summary The effects of heat-killed Lactobacillus paracasei MCC1849 (LAC-Shield™) on the common cold and mood states during winter in healthy adults were evaluated in a randomised, double-blind, placebo-controlled trial. In a subgroup of subjects susceptible to the common cold, the incidence and symptom scores of the common cold significantly improved in the 10 billion cell MCC1849-intake group. The results of this study indicate that paraprobiotic L. paracasei MCC1849 has the potential to improve resistance to common cold infections and maintain a desirable mood state, even under mental stress conditions.
Title Effects of Bifidobacterium breve B-3 on body fat reductions in pre-obese adults: A randomized, double-blind, placebo-controlled trial
Author J.Minami, N.Iwabuchi, M.Tanaka, K.Yamauchi, J.Xiao, F.Abe, and N.Sakane* *National Hospital Organization Kyoto Medical Center
Journal Bioscience of Microbiota, Food and Health 37(3): 67-75(2018)
Summary The effects of the consumption of Bifidobacterium breve B-3 on body fat reduction in healthy pre-obese (25 ≤ BMI < 30) adults were investigated in a double-blind, placebo-controlled trial. Body fat mass was significantly lower in the B-3 group than in the placebo group. Waist circumference in the B-3 group was significantly lower at week 12 than at the baseline.
Title Genomic diversity and distribution of Bifidobacterium longum subsp. longum across the human lifespan
Author T.Odamaki, F.Bottacini*, K.Kato, E.Mitsuyama, K.Yoshida, A.Horigome, JZ.Xiao, and D.van Sinderen*
*APC Microbiome Institute and School of Microbiology, National University of Ireland
Journal Scientific Reports 8(1): Art. 85 (2018)
Summary We performed a comparative genome analysis of Bifidobacterium longum subsp. longum to unravel the reason why this subspecies are widely distributed across all generation. As results, a broad distribution of them across the human lifespan is due to a high transfer level between family members. We furthermore identified a variety of genes which could enhance their adaptation and increase competitiveness in the gut environment, enriched in genomes corresponding to specific ages.

Production technology

Title The characteristics of the manufacturing method and the attempt to expand the use of long life tofu products
Author T.Miyai
Journal Food Chemical 35(6): 63-65 (2019)
Summary Tofu was legally required to be sold in cold conditions for a long time, but the law was revised in 2018. Following that revision, we launched long life tofu products nationwide which can be stored at room temperature in the home delivery market on January in 2019. In this review, I introduced the differences compared with traditional tofu including the manufacturing method and packaging.

Analytical technology

Title Pep-MS assay: Protease hydrolysis assay system using photo-cleavable peptide array and mass spectrometer
Author M.Kurimoto, K.Shimizu*1, H.Ochi, F.Abe, and H.Honda*1,2
*1Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, *2Innovative Research Center for Preventative Medical Engineering, Nagoya University
Journal Journal of Bioscience and Bioengineering 128(2): 156-161(2019)
Summary We have developed a "Pep-MS assay" system that could be used to comprehensively clarify the positions of protease cleavage. The Pep-MS assay can identify a lot of enzyme cleavage sites at once, and the data obtained using this technology is useful for efficiently producing bioactive peptides.

Hygiene

Title Effect of overhead irrigation with slightly acidic hypochlorous acid water on reducing anthracnose infection of Cultivars Kirapika and Benihoppe strawberry seedlings
Author T.Nakamura, T.Ikari*, M.Tanaka, K.Yamauchi, and F.Abe
*Shizuoka Prefectural Research Institute of Agriculture and Forestry
Journal Annual Report of the Kanto-Tosan Plant Protection Society, No. 65 pp.32-34 (2018)
Summary Use of slightly acidic hypochlorous acid water (PURESTER-generated water) to irrigate strawberry seedlings revealed that it had an effect for reducing infection with anthracnose, a major disease with a strong bearing on cultivation. In addition, because there was no detectable adverse influence on growth, it is thought that slightly acidic hypochlorous acid water could be effectively used for irrigation of strawberry seedlings.

Functional Ingredients

Title Effects of Aloe sterol supplementation on postprandial blood glucose elevation after OGTT in healthy subjects: A 12-week double-blind randomized placebo-controlled trial
Author Eriko Misawa, Daisuke Ochi, Koji Nomaguchi, Marie Saito, Kazumi Nabeshima, Miyuki Tanaka, Izumi Kakiuchi*1, Kyoko Kiyosawa*1, Mitsunaga Miyasaka*1, Masahiko Nakamura*2, Naoki Sakane*3
*1 Department of Nursing, Matsumoto Junior College, *2 Department of Neurosurgery, Matsumoto City Hospital, *3Division of Preventive Medicine, Clinical Research Institute, National Hospital Organization Kyoto Medical Center
Journal Japanese Pharmacology & Therapeitics 49(7): 1139-1147 (2021)
Summary In this study, we conducted clinical trial to evaluate the effects of Aloe sterols on glucose metabolism. Healthy subjects were allocated to an Aloe sterol group or a placebo group, and ingested Aloe sterol capsules or placebo capsules daily for 12 weeks. An oral glucose tolerance test (OGTT) was carried out at 0 and 12 weeks. As results, the changes at 0 and 12 weeks in blood glucose levels at 60 minutes after OGTT and blood glucose AUC were significantly lower in the Aloe sterol group than placebo group, even though there was no difference in blood insulin levels. From these results, it is considered that dietary supplementation with Aloe sterol has the beneficial effect of lowering postprandial blood glucose levels by improving insulin sensitivity in healthy individuals.
Title Effects of bovine lactoferrin on plasmacytoid dendritic cells in peripheral blood of healthy adults
Author S.Kubo, M.Miyakawa, A.Tada, H.Oda, H.Motobayashi*1, S.Iwabuchi*2, S.Tamura*3, M.Tanaka, and S.Hashimoto*2
*1Second Department of Surgery, Wakayama Medical University, *2Department of Molecular Pathophysiology, Wakayama Medical University, *3Department of Hematology/Oncology, Wakayama Medical University.
Journal Japanese Pharmacology & Therapeitics 49(8):1289-1293 (2021)
Summary Bovine lactoferrin (LF) enhanced the expression of a co-stimulatory molecule on plasmacytoid dendritic cells (pDCs) in peripheral blood mononuclear cells derived from healthy adults. Therefore, activation of pDCs may mediate the immunomodulatory effects of LF.
Title Safety evaluation of high‐dose intake of casein‐derived peptide Met‐Lys‐Pro in healthy adults: A randomized, double‐blind, placebo‐controlled trial
Author N.Yuda, M.Tanaka, M.Tokushima*, and F.Abe
*Maebashi North Hospital
Journal Food Science & Nutrition 9(2): 662-671 (2021)
Summary We conducted a 4-week, randomized, double-blind, placebo-controlled trial to evaluate the safety of high-dose intake of the casein-derived peptide Met-Lys-Pro (MKP) in healthy adults. The results showed that there were no clinical safety concerns associated with high-dose intake of MKP. The present study suggested that MKP supplementation is safe.
Title Lactulose ingestion causes an increase in the abundance of gut-resident bifidobacteria in Japanese women: a randomised, double-blind, placebo-controlled crossover trial
Author Y.Sakai, H.Hamano, H.Ochi, F.Abe, K.Masuda*1, and H.Iino*2
*1Department of Food Science and Nutrition, Faculty of Life and Environmental Sciences, Showa Women's University, *2Life Sciences for Living Systems, Graduate School, Showa Women's University
Journal Beneficial Microbes 12:43-53 (2021)
Summary In a study of 60 healthy Japanese women, the 2 g/day lactulose ingestion for 2 weeks significantly increased the human gut-resident bifidobacteria: B. adolescentis, B. catenulatum,, and B. longum. However, it did not significantly affect the diversity of the intestinal microbiota.
Title Effect of the casein-derived peptide Met-Lys-Pro on cognitive function in community-dwelling adults without dementia: A randomized, double-blind, placebo-controlled trial
Author N.Yuda, M.Tanaka, K.Yamauchi, F.Abe, I.Kakiuchi*1, K.Kiyosawa*1, M.Miyasaka*1, N.Sakane*2, and M.Nakamura*3
*1Department of Nursing, Matsumoto Junior College, *2Division of Preventive Medicine, Clinical Research Institute, National Hospital Organization Kyoto Medical Center, *3Matsumoto City Hospital
Journal Clinical Interventions in Aging 15: 743-754 (2020)
Summary We conducted a 24-week, randomized, double-blind, placebo-controlled trial to investigate the ability of the casein-derived peptide Met-Lys-Pro (MKP) to improve cognitive function in a population of community-dwelling adults without dementia. The present study showed that MKP supplementation may have the potential to improve orientation.
Title Effects of casein hydrolysate ingestion on thermoregulatory responses in healthy adults during exercise in heated conditions: A randomized crossover trial 
Author Y.Sakata, C.Yoshida*, Y.Fujiki*, Y.Matsunaga, H.Nakamura, T.Shimizu, Y.Takeda, and T.Amano*
*Laboratory for Exercise and Environmental Physiology, Faculty of Education, Niigata University
Journal Nutrients 12(3): 867 (2020)
Summary The effects of casein hydrolysate ingestion on thermoregulatory responses during exercise in heated conditions were evaluated in a randomized, double-blind, placebo-controlled crossover trial. The results of this study indicate that casein hydrolysate ingestion before exercise increased skin temperature and sweating.
Title Effects of lactoferrin-fortified formula on acute gastrointestinal symptoms in children aged 12-32 months: A randomized, double-blind, placebo-controlled trial
Author N.Motoki*, M.Mizuki*, T.Tsukahara*, M.Miyakawa, S.Kubo, H.Oda, M.Tanaka, K.Yamauchi, F.Abe, and T.Nomiyama*
*Department of Preventive Medicine and Public Health, Shinshu University School of Medicine
Journal Frontiers in Pediatrics 8:233 (2020)
Summary We investigated the effect of lactoferrin (LF)-fortified formula on gastrointestinal and respiratory symptoms in children aged 1-2 years. This investigation demonstrated that ingestion of LF could decrease the prevalence of acute gastrointestinal symptoms and lower the total days of acute respiratory symptoms in children.
Title Effect of tablets containing lactoferrin and lactoperoxidase on gingival health in adults: A randomized, double-blind, placebo-controlled clinical trial
Author M.Nakano, A.Yoshida*1, H.Wakabayashi, M.Tanaka, K.Yamauchi, F.Abe, and Y.Masuda*2
*1Department of Oral Microbiology, Faculty of Dentistry, Matsumoto Dental University *2Institute for Oral Science, Graduate school of Oral Medicine, Matsumoto Dental University
Journal Journal of Periodontal Research 54(6): 702-708 (2019)
Summary We conducted the clinical trial to evaluate the influence of long‐term use of LF and LPO‐containing tablets on gingival health and oral health‐related quality of life in healthy subjects. Long-term ingestion of tablets containing lactoferrin and lactoperoxidase significantly improved gingival inflammation and oral health‐related quality of life compared with the placebo.
Title Effects of milk casein hydrolysate intake on the body composition of healthy women undertaking habitual exercise in fitness clubs: A randomized open controlled pilot study
Author F.Saito, H.Nakamura, Y.Takeda, K.Watanabe*1, and T.Tominaga*2
*1TANITA Corporation、*2Tominaga Clinic
Journal Japanese Journal of Health Promotion 21(2):145-152 (2019)
Summary We examined the effects of milk casein hydrolysate intake on the body composition of healthy women doing habitual exercise in fitness clubs. In conclusion, no obvious effects of the casein hydrolysate intake on the body composition could be confirmed. However, it was suggested that there may be some influence on the body composition or energy metabolism in a certain populations.
Title Novel health function of Aloe vera gel-derived phytosterols and their application in functional foods(JSNFS Award for Technology and Innovation (2018))
Author E.Misawa, M.Tanaka, F.Abe, K.Yamauchi, M.Saito, and K.Nabeshima
Journal Journal of Japan Society of Nutrition and Food Sciences 72(4): 141-145 (2019)
Summary We have identified five phytosterols (Aloe sterol) obtained from Aloe vera gel as functional ingredients. We investigated the effects on skin function by basic and clinical studies. From these studies, it was confirmed that the usefulness of Aloe sterol on maintenance and promotion of skin health as functional food material.
Title Prebiotic effect of two grams of lactulose in healthy Japanese women: a randomised, double-blind, placebo-controlled crossover trial
Author Y.Sakai, N.Seki, H.Hamano, H.Ochi, F.Abe, K.Masuda*1, and H.Iino*2
*1Department of Food Science and Nutrition, Faculty of Life and Environmental Sciences, Showa Women’s University, *2Life Sciences for Living Systems, Graduate School, Showa Women’s University
Journal Beneficial Microbes 10(6): 629-639 (2019)
Summary In a randomised, double-blind crossover trial with 60 healthy Japanese women, the defaecation frequency, faecal consistency, faecal volume, and the number and percentage of Bifidobacterium in faeces were significantly higher during 2 g/day lactulose than placebo, but flatulence was unchanged.
Title A study of the prebiotic effect of lactulose at low dosages in healthy Japanese women
Author Y.Sakai, N.Seki, H.Hamano, H.Ochi, F.Abe, F.Shimizu*1, K.Masuda*1, and H.Iino*2
*1Department of Food Science and Nutrition, Faculty of Life and Environmental Sciences, Showa Women’s University, *2Life Science for Living System, Graduate School, Showa Women’s University,
Journal Bioscience of Microbiota, Food and Health 38(2): 69-72 (2019)
Summary We assessed changes in defaecation frequency following ingestion of 1, 2, or 3 g/day of lactulose for 2 weeks. This was an open-label, before-after trial that enrolled 26 healthy Japanese women. The defaecation frequency, number of defaecation days, and number of faecal bifidobacteria increased significantly compared with before ingestion of 1, 2, and 3 g/day of lactulose.
Title Casein materials show different digestion patterns using an in vitro gastrointestinal model and different release of glucagon-like peptide-1 by enteroendocrine GLUTag cells
Author Y.Komatsu, Y.Wada, H.Izumi, T.Shimizu, Y.Takeda, T.Hira*, and H.Hara*
*Research Faculty of Agriculture, Hokkaido University
Journal Food Chemistry 277: 423-431 (2019)
Summary We compared protein digestibility and glucagon-like peptide-1 (GLP-1) -releasing activities of micellar casein concentrate (MCC) and sodium caseinate (SCN). The digestibility of MCC was higher than that of SCN. A comparison of GLP-1-releasing activities showed that MCC induced GLP-1 secretion to a greater extent than SCN.
Title Investigation of potent odorants generated during the production
Author H.Nakada, M.Ohata*1,2, M.Hosaka*1, H.Ochi, F.Abe, and K.Arihara*1
*1Kitasato University, *2Nihon University
Journal Animal Science Journal 89(9):1348-1354(2018)
Summary We evaluated predominant odorants of Whey protein hydrolysates(WPH), and our results showed that difference of odor characteristics between whey protein concentrate(WPC) and WPH, and three odor-active compounds contributed to the odor of WPH. Additionally, this study suggests that specific free amino acids are important precursors for generation of these compounds in manufacturing process of WPH.
Title Antihypertensive effect of the casein-derived peptide Met-Lys-Pro in individuals with high-normal blood pressure or grade 1 hypertension-A randomized, double-blind, placebo-controlled, parallel-group trial-
Author N.Yuda, M.Tanaka, A.Yamada, D.Ochi, K.Yamauchi, F.Abe, and N.Sakane*
*National Hospital Organization Kyoto Medical Center
Journal Japan Pharmacology & Therapeutics 46(4):529-37(2018)
Summary Antihypertensive effect of the casein-derived peptide Met-Lys-Pro (MKP) was confirmed by clinical trial. Daily MKP ingestion is a effective systolic blood pressure lowering treatment for people with mildly high blood pressure.
Title Blood pressure-lowering effects of casein-derived peptide met-lys-pro in subjects with high-normal blood pressure and grade 1 hypertension - A randomized, double-blind, placebo-controlled, parallel-group, trial-
Author D.Ochi, A.Yamada, K.Yamauchi, F.Abe, and Y.Tho*
*Aiwa Clinic, Latvia
Journal Japanese pharmacology & therapeutics 45(10): 1637-1648 (2017)
Summary We evaluated the effect of Met-Lys-Pro(MKP) in lowering blood pressure in subjects with high-normal blood pressure and grade 1 hypertension in a randomized, double-blind, placebo-controlled, parallel-group trial for 12 weeks.
The result showed that 100 μg MKP has a beneficial effect on lowering systolic and diastolic blood pressure.
Title Effects of lactoferrin and lactoperoxidase-containing food on the oral microbiota of older individuals
Author M.Nakano, H.Wakabayashi, H.Sugahara, T.Odamaki, K.Yamauchi, F.Abe, JZ.Xiao, K.Murakami*, K.Ishikawa*, and S.Hironaka*
*Department of Special Needs Dentistry, Division of Hygiene and Oral Health, Showa University School of Dentistry
Journal Microbiology and Immunology 61(10): 416-426 (2017)
Summary The long-term ingestion of lactoferrin and lactoperoxidase-containing tablets promotes a shift from a gram-negative-dominated unhealthy community to a gram-positive-dominated healthy community in the oral microbiota.
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